912545-86-9-SAG SAG

SAG

For research use only. Not for therapeutic Use.

  • CAT Number: I005440
  • CAS Number: 912545-86-9
  • Molecular Formula: C28H28ClN3OS
  • Molecular Weight: 490.1
  • Purity: ≥95%
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SAG (Cat.No:I005440) is a potent Smoothened (Smo) receptor agonist (Kd = 59 nM); potently activates the Hedgehog signaling pathway in Shh-light 2 cells (EC50 ~ 3 nM)
in vitro: The binding of BODIPY-cyclopamine to the Smo-deletion mutants was inhibited by 150 nM SAG to an extent similar to that observed with cells expressing WT Smo. SAG may interact not only with Smo, but also with a cellular effector of Smo activation, thereby inducing Hh pathway activation by facilitating the association of these two proteins at optimal SAG concentrations


Catalog Number I005440
CAS Number 912545-86-9
Synonyms

3-chloro-N-((1r,4r)-4-(methylamino)cyclohexyl)-N-(3-(pyridin-4-yl)benzyl)benzo[b]thiophene-2-carboxamide

Molecular Formula C28H28ClN3OS
Purity ≥95%
Target Hedgehog
Solubility DMSO: ≥ 38 mg/mL
Storage Store at -20°C
IC50 59 nM (Ki)
IUPAC Name 912545-86-9
InChI InChI=1S/C28H28ClN3OS/c1-30-22-9-11-23(12-10-22)32(28(33)27-26(29)24-7-2-3-8-25(24)34-27)18-19-5-4-6-21(17-19)20-13-15-31-16-14-20/h2-8,13-17,22-23,30H,9-12,18H2,1H3
InChIKey VFSUUTYAEQOIMW-YHBQERECSA-N
SMILES CNC1CCC(CC1)N(CC2=CC(=CC=C2)C3=CC=NC=C3)C(=O)C4=C(C5=CC=CC=C5S4)Cl
Reference

</br>1:SU-E-T-88: Evaluating Gantry Sag on Linear Accelerators and Introducing an MLC-Based Compensation Strategy. Du W, Gao S, Wang X, Kudchadker R.Med Phys. 2012 Jun;39(6Part11):3722-3723. doi: 10.1118/1.4735145. PMID: 28517157 </br>2:Early γ-irradiation and subsequent storage of red cells in SAG-M additive solution potentiate energy imbalance, microvesiculation and susceptibility to stress-induced apoptotic cell death. Qadri SM, Chen D, Schubert P, Devine DV, Sheffield WP.Vox Sang. 2017 Apr 5. doi: 10.1111/vox.12518. [Epub ahead of print] PMID: 28378415 </br>3:SAG-QC: quality control of single amplified genome information by subtracting non-target sequences based on sequence compositions. Maruyama T, Mori T, Yamagishi K, Takeyama H.BMC Bioinformatics. 2017 Mar 4;18(1):152. doi: 10.1186/s12859-017-1572-5. PMID: 28259144 Free PMC Article</br>4:Combining Smoothened Agonist (SAG) and NEL-like protein-1 (NELL-1) Enhances Bone Healing. Lee S, Wang C, Pan HC, Shrestha S, Meyers C, Ding C, Shen J, Chen E, Lee M, Soo C, Ting K, James AW.Plast Reconstr Surg. 2017 Feb 13. doi: 10.1097/PRS.0000000000003367. [Epub ahead of print] PMID: 28198775 </br>5:Influence of Irradiation on Leukodepleted Small Unit Red Blood Cell (RBC) Bags for Infant Transfusion in Additive Solution SAG-M. Hauck-Dlimi B, Schiffer K, Eckstein R, Strobel J, Zimmermann R.Clin Lab. 2016 Jul 1;62(7):1295-1301. doi: 10.7754/Clin.Lab.2015.151127. PMID: 28164645 </br>6:Depletion of SAG/RBX2 E3 ubiquitin ligase suppresses prostate tumorigenesis via inactivation of the PI3K/AKT/mTOR axis. Tan M, Xu J, Siddiqui J, Feng F, Sun Y.Mol Cancer. 2016 Dec 12;15(1):81. PMID: 27955654 Free PMC Article</br>7:SAG/RBX2 E3 ligase complexes with UBCH10 and UBE2S E2s to ubiquitylate β-TrCP1 via K11-linkage for degradation. Kuang P, Tan M, Zhou W, Zhang Q, Sun Y.Sci Rep. 2016 Dec 2;6:37441. doi: 10.1038/srep37441. PMID: 27910872 Free PMC Article</br>8:Can inorganic phosphate explain sag during unfused tetanic contractions of skeletal muscle? Smith IC, Bellissimo C, Herzog W, Tupling AR.Physiol Rep. 2016 Nov;4(22). pii: e13043. PMID: 27884960 Free PMC Article</br>9:Preaxial polydactyly following early gestational exposure to the smoothened agonist, SAG, in C57BL/6J mice. Fish EW, Parnell SE, Sulik KK, Baker LK, Murdaugh LB, Lamson D, Williams KP.Birth Defects Res. 2017 Jan 20;109(1):49-54. doi: 10.1002/bdra.23571. PMID: 27801979 </br>10:Reply: Does the Eyebrow Sag with Aging? An Anthropometric Study of 95 Caucasians from 20 to 79 Years of Age. Bruneau S, Foletti JM.Plast Reconstr Surg. 2016 Nov;138(5):941e-942e. No abstract available. PMID: 27783020

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