Sepin-1

• CAT Number: I036223
• CAS Number: 163126-81-6
• Molecular Formula: C9H9N3O4
• Molecular Weight: 223.19
• Purity: ≥95%
• Synonyms: 2,2-dimethyl-5-nitro-3-oxidobenzimidazol-1-ium 1-oxide
• InChI: InChI=1S/C9H9N3O4/c1-9(2)10(13)7-4-3-6(12(15)16)5-8(7)11(9)14/h3-5H,1-2H3
• InChIKey: YLVSVJDCTDBERH-UHFFFAOYSA-N
• SMILES: CC1(N(C2=C([N+]1=O)C=CC(=C2)[N+](=O)[O-])[O-])C

Sepin-1 is a potent separase inhibitor with an IC50 value of 14.8 µM. Sepin-1 inhibits cell proliferation, migration and wound healing. Sepin-1 decreases the expression of FoxM1 protein and mRNA level. Sepin-1 shows anti-tumor activity[1][2].
Sepin-1 (0, 20, 40 µM; 24 h) inhibits cell migration and wound healing, and causes DNA damage[1].
Sepin-1 (0, 10, 20, 40 µM; 24 h) decreases the expression of FoxM1 protein and mRNA level in a dose-dependent manner[1].
Sepin-1 (0, 10, 20, 40 µM; 24 h) inhibits the expression of Plk1, Cdk1, pericentrin, Aurora, and Lamin B1 protein levels[1].
Sepin-1 (10 mg/kg; i.v.; daily for 5 days a week for 3 weeks) shows anti-tumor activity in mice[2].

Reference

[1]. Zhang N, et al. Separase Inhibitor Sepin-1 Inhibits Foxm1 Expression and Breast Cancer Cell Growth. J Cancer Sci Ther. 2018;10(3):517.
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[2]. Zhang N, et al. Identification and Characterization of Separase Inhibitors (Sepins) for Cancer Therapy. J Biomol Screen. 2014 Jul;19(6):878-89.
 [Content Brief]