Small Cardioactive Peptide B (SCPB)

• CAT Number: I020204
• CAS Number: 84746-43-0
• Molecular Formula: C52H80N14O11S2
• Molecular Weight: 1141.41
• Purity: ≥95%
• Synonyms: (2S)-2-[[(2S)-2-amino-4-methylsulfanylbutanoyl]amino]-N-[(2S)-1-[[(2S)-1-[[(2S)-1-[[(2S)-1-[(2S)-2-[[(2S)-1-[[(2S)-1-amino-4-methylsulfanyl-1-oxobutan-2-yl]amino]-5-(diaminomethylideneamino)-1-oxopentan-2-yl]carbamoyl]pyrrolidin-1-yl]-1-oxo-3-phenylpropan-2-yl]amino]-1-oxopropan-2-yl]amino]-4-methyl-1-oxopentan-2-yl]amino]-3-(4-hydroxyphenyl)-1-oxopropan-2-yl]butanediamide
• InChI: InChI=1S/C52H80N14O11S2/c1-29(2)25-37(63-48(74)38(26-32-15-17-33(67)18-16-32)64-49(75)39(28-42(54)68)62-45(71)34(53)19-23-78-4)47(73)59-30(3)44(70)65-40(27-31-11-7-6-8-12-31)51(77)66-22-10-14-41(66)50(76)61-36(13-9-21-58-52(56)57)46(72)60-35(43(55)69)20-24-79-5/h6-8,11-12,15-18,29-30,34-41,67H,9-10,13-14,19-28,53H2,1-5H3,(H2,54,68)(H2,55,69)(H,59,73)(H,60,72)(H,61,76)(H,62,71)(H,63,74)(H,64,75)(H,65,70)(H4,56,57,58)/t30-,34-,35-,36-,37-,38-,39-,40-,41-/m0/s1
• InChIKey: IDATZUYVZKJKRE-SXMXEBDWSA-N
• SMILES: CC(C)CC(C(=O)NC(C)C(=O)NC(CC1=CC=CC=C1)C(=O)N2CCCC2C(=O)NC(CCCN=C(N)N)C(=O)NC(CCSC)C(=O)N)NC(=O)C(CC3=CC=C(C=C3)O)NC(=O)C(CC(=O)N)NC(=O)C(CCSC)N

Small Cardioactive Peptide B (SCPB), a neurally active peptide, stimulates adenylate cyclase activity in particulate fractions of both heart and gill tissues with EC50s of 0.1 and 1.0 μM, respectively.
Small cardioactive peptide B (SCPB) is a neurally active peptide endogenous to Aplysia. Small cardioactive peptide B (SCPB) possesses cardioexcitatory effects in Aplysia and reported a threshold concentration of 0.01 nM for both native and synthetic Small cardioactive peptide B (SCPB) stimulated effects on the isolated heart. Effects of Small Cardioactive Peptide B (SCPB) on the physiology of the isolated heart and gill preparations from the mollusc Aplysia californica were examined. In addition, the effects of Small Cardioactive Peptide B (SCPB) and FMRFamide (Phe-Met-Arg-Phe-NH2) on adenylate cyclase activity are compared in particulate fractions of heart and gill tissues, respectively. Small Cardioactive Peptide B (SCPB) is found to exert dose-dependent, reversible changes in cardiac activity when perfused through the isolated heart. The EC50 values effecting changes in heart rate and force of contraction are 0.03 and 0.3 nM, respectively; minimum concentrations find to effect changes in heart rate and force of contraction are normally 0.001 and 1 pM, respectively. When perfused through the isolated gill, Small Cardioactive Peptide B (SCPB) is found to suppress the gill withdrawal response amplitude with a threshold concentration of 0.01 pM and an EC50 value of 0.03 nM. Suppression of the gill withdrawal response amplitude by Small Cardioactive Peptide B (SCPB)is found to be dose dependent and reversible up to a concentration of 1nM. At higher concentrations, the suppression tended to persist irreversibly. Small Cardioactive Peptide B (SCPB) stimulates adenylate cyclase activity in particulate fractions of both heart and gill tissues with an EC50 of 0.1 and 1.0μM, respectively[1].

Reference

[1]. Cawthorpe DR, et al. The effects of small cardioactive peptide B on the isolated heart and gill of Aplysia californica. Can J Physiol Pharmacol. 1985 Aug;63(8):918-24.
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