Sp-cAMPS sodium salt

• CAT Number: I011728
• CAS Number: 142439-95-0
• Molecular Formula: C10H11N5NaO5PS
• Molecular Weight: 367.25
• Purity: ≥95%

Sp-cAMPS sodium salt, a cAMP analog, is potent activator of cAMP-dependent PKA I and PKA II. Sp-cAMPS sodium salt is also a potent, competitive phosphodiesterase (PDE3A) inhibitor with a Ki of 47.6 µM. Sp-cAMPS sodium salt binds the PDE10 GAF domain with an EC50 of 40 μM[1][2][3].
Treatment of hepatocytes with Sp-cAMPS sodium salt, the stimulatory diastereomer of adenosine cyclic 3′,5′-phosphorothioate, mimics the response seen with glucagon. The glucagon-stimulated increases in the level of Ca2+ can be mimicked by Sp-cAMPS sodium salt[4].
In chronic alcohol consumption (CAC) mice, direct infusion of the Sp-cAMPS (1 µg/µL) sodium salt into the prefrontal cortex significantly improves or impairs, respectively, working memory performance in withdrawn and water animals[5].

Reference

[1]. Su H Hung, et al. A new nonhydrolyzable reactive cAMP analog, (Sp)-adenosine-3′,5′-cyclic-S-(4-bromo-2,3-dioxobutyl)monophosphorothioate irreversibly inactivates human platelet cGMP-inhibited cAMP phosphodiesterase. Bioorg Chem. 2002 Feb;30(1):16-31.
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[2]. L Y Wang, et al. Regulation of kainate receptors by cAMP-dependent protein kinase and phosphatases. Science. 1991 Sep 6;253(5024):1132-5.
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[3]. Ronald Jäger, et al. Activation of PDE10 and PDE11 phosphodiesterases. J Biol Chem. 2012 Jan 6;287(2):1210-9.
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[4]. P A Connelly,et al. A study of the mechanism of glucagon-induced protein phosphorylation in isolated rat hepatocytes using (Sp)-cAMPS and (Rp)-cAMPS, the stimulatory and inhibitory diastereomers of adenosine cyclic 3′,5′-phosphorothioate. J Biol Chem. 1987 Mar 25;262(9):4324-32.
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[5]. G Dominguez, et al. Rescuing prefrontal cAMP-CREB pathway reverses working memory deficits during withdrawal from prolonged alcohol exposure. Brain Struct Funct. 2016 Mar;221(2):865-77.
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