For research use only. Not for therapeutic Use.
Suc-<wbr></wbr>AAPF-<wbr></wbr><em>p</em>NA is a chromogenic substrate that can be cleaved by cathepsin G (K<sub>m</sub> = 1.7 mM), subtilisins, chymotrypsin (K<sub>m</sub> = 60 µM), chymase (K<sub>m</sub> = 4 mM), and cyclophilin, but not neutrophil elastase. Release of <em>p-<wbr></wbr></em>nitroanilide is monitored at 405-<wbr></wbr>410 nm. This substrate can be used for inhibitor screening and kinetic analysis.
| Catalog Number | R064945 |
| CAS Number | 70967-97-4 |
| Synonyms | Succinyl-Ala-Ala-Pro-Phe-p-nitroanilide |
| Molecular Formula | C30H36N6O9 |
| Purity | ≥95% |
| Storage | -20°C |
| InChI | InChI=1S/C30H36N6O9/c1-18(31-25(37)14-15-26(38)39)27(40)32-19(2)30(43)35-16-6-9-24(35)29(42)34-23(17-20-7-4-3-5-8-20)28(41)33-21-10-12-22(13-11-21)36(44)45/h3-5,7-8,10-13,18-19,23-24H,6,9,14-17H2,1-2H3,(H,31,37)(H,32,40)(H,33,41)(H,34,42)(H,38,39)/t1 |
| InChIKey | LKDMKWNDBAVNQZ-WJNSRDFLSA-N |
| SMILES | OC(CCC(N[C@@H](C)C(N[C@@H](C)C(N1CCC[C@H]1C(N[C@@H](CC2=CC=CC=C2)C(NC3=CC=C([N+]([O-])=O)C=C3)=O)=O)=O)=O)=O)=O |
| Reference | 1.Nakajima, K., and Powers, J.C. Mapping the extended substrate binding site of cathepsin G and human leukocyte elastase. The Journal of Biological Chemisty 254(10), 4027-4032 (1979). |
