Suc-Ala-Ala-Phe-AMC

• CAT Number: I043462
• CAS Number: 71973-79-0
• Molecular Formula: C29H32N4O8
• Molecular Weight: 564.59
• Purity: ≥95%
• Synonyms: 4-[[(2S)-1-[[(2S)-1-[[(2S)-1-[(4-methyl-2-oxochromen-7-yl)amino]-1-oxo-3-phenylpropan-2-yl]amino]-1-oxopropan-2-yl]amino]-1-oxopropan-2-yl]amino]-4-oxobutanoic acid
• IUPAC Name: 4-[[1-[[1-[[1-[(4-methyl-2-oxochromen-7-yl)amino]-1-oxo-3-phenylpropan-2-yl]amino]-1-oxopropan-2-yl]amino]-1-oxopropan-2-yl]amino]-4-oxobutanoic acid
• InChI: InChI=1S/C29H32N4O8/c1-16-13-26(37)41-23-15-20(9-10-21(16)23)32-29(40)22(14-19-7-5-4-6-8-19)33-28(39)18(3)31-27(38)17(2)30-24(34)11-12-25(35)36/h4-10,13,15,17-18,22H,11-12,14H2,1-3H3,(H,30,34)(H,31,38)(H,32,40)(H,33,39)(H,35,36)
• InChIKey: HHPVJKZZYOXPLH-UHFFFAOYSA-N
• SMILES: CC1=CC(=O)OC2=C1C=CC(=C2)NC(=O)C(CC3=CC=CC=C3)NC(=O)C(C)NC(=O)C(C)NC(=O)CCC(=O)O

Suc-Ala-Ala-Phe-AMC（Cat No.:I043462）is a synthetic peptide substrate commonly used in biochemical assays to measure the activity of enzymes such as proteases. The peptide consists of an N-terminal succinyl group (Suc), followed by alanine (Ala), phenylalanine (Phe), and a 7-amino-4-methylcoumarin (AMC) group at the C-terminus. Upon enzymatic cleavage of the peptide bond between Ala and Phe, AMC is released, emitting fluorescence that can be measured to quantify enzyme activity. This substrate is widely used in research involving protease activity, such as screening for inhibitors or studying protease function in various biological processes.