t6A

• CAT Number: I036933
• CAS Number: 24719-82-2
• Molecular Formula: C15H20N6O8
• Molecular Weight: 412.35
• Purity: 98%
• Synonyms: t6A; t-6-A; t 6 A; t(6)A; N6-Threonylcarbamoyladenosine; N-(6-Nebularinylcarbamoyl)threonine
• Solubility: Soluble in DMSO
• Appearance: Solid powder
• Storage: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
• IUPAC Name: ((9-((2R,3R,4S,5R)-3,4-dihydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-9H-purin-6-yl)carbamoyl)-L-threonine
• InChI: InChI=1S/C15H20N6O8/c1-5(23)7(14(26)27)19-15(28)20-11-8-12(17-3-16-11)21(4-18-8)13-10(25)9(24)6(2-22)29-13/h3-7,9-10,13,22-25H,2H2,1H3,(H,26,27)(H2,16,17,19,20,28)/t5-,6-,7+,9-,10-,13-/m1/s1
• InChIKey: UNUYMBPXEFMLNW-DWVDDHQFSA-N
• SMILES: O[C@@H]1[C@@H](CO)O[C@@H](N2C=NC3=C2N=CN=C3NC(N[C@@H]([C@H](O)C)C(O)=O)=O)[C@@H]1O

N6-Threonylcarbamoyladenosine (t6A)(CAT: I036933) is a universally conserved and essential modified nucleoside located at position 37 in transfer RNAs (tRNAs) that decode ANN codons across all domains of life—Archaea, Bacteria, and Eukarya. This modification enhances translational fidelity by stabilizing codon-anticodon pairing and preventing frameshifting during protein synthesis. t6A is synthesized through a multistep enzymatic pathway involving threonine, ATP, and carbamoyl phosphate. Its critical role in maintaining the efficiency and accuracy of protein translation makes t6A a vital focus in Genetic Research and Molecular Biology, particularly in studies related to gene expression regulation and translation mechanisms.