Thymidine 3',5'-diphosphate tetrasodium

• CAT Number: I041823
• CAS Number: 118675-87-9
• Molecular Formula: C10H12N2Na4O11P2
• Molecular Weight: 490.12
• Purity: ≥95%
• Synonyms: tetrasodium;[(2R,5R)-5-(5-methyl-2,4-dioxopyrimidin-1-yl)-2-(phosphonatooxymethyl)oxolan-3-yl] phosphate
• InChI: InChI=1S/C10H16N2O11P2.4Na/c1-5-3-12(10(14)11-9(5)13)8-2-6(23-25(18,19)20)7(22-8)4-21-24(15,16)17;;;;/h3,6-8H,2,4H2,1H3,(H,11,13,14)(H2,15,16,17)(H2,18,19,20);;;;/q;4*+1/p-4/t6?,7-,8-;;;;/m1..../s1
• InChIKey: VDPDGAPWPLBFIC-TVQGIFJUSA-J
• SMILES: CC1=CN(C(=O)NC1=O)C2CC(C(O2)COP(=O)([O-])[O-])OP(=O)([O-])[O-].[Na+].[Na+].[Na+].[Na+]

Thymidine 3′,5′-diphosphate (Deoxythymidine 3′,5′-diphosphate) tetrasodium is a selective inhibitor of staphylococcal nuclease and tudor domain containing 1 (SND1, the MicroRNA regulatory complex RISC subunit) and [3,5-2H2] tyrosyl nuclease. Thymidine 3′,5′-diphosphate tetrasodium has anti-tumor activity and can also be used as a catalyst in biochemical reactions[1][2].
Thymidine 3′,5′-diphosphate tetrasodium (200 µM; 18 h) significantly reduces the expression level of p65 and p65 nuclear translocation in WT and Alb/SND1 (specific transgenic mouse overexpressing SND1) hepatocytes by inhibiting staphylococcal nuclease and tudor domain containing 1 (SND1) enzyme activity. Thymidine 3′,5′-diphosphate tetrasodium inhibits the spherical formation of WT and Alb/SND1 hepatocytes[1].
Thymidine 3′,5′-diphosphate tetrasodium (0.8 mg/kg; i.p.; twice a week for 4 weeks) has insignificant effect on serum liver enzymes (AST, ALT, AP), total protein (TP), albumin (Alb), and globulin (Glo) in WT B6/CBA mice[1].
Thymidine 3′,5′-diphosphate tetrasodium (0.8 mg/kg and 1.6 mg/kg; i.v.; twice a week for 4 weeks) significantly inhibits tumor growth in WT B6/CBA mice[1].
Thymidine 3′,5′-diphosphate tetrasodium (0.8, 0.16 and 0.32 mg/kg; s.c.; twice a week for 4 weeks) inhibits tumor growth, inflammatory reaction and the expression of tumor initiating cells (TIC) markers in adult male NSG mice. Thymidine 3′,5′-diphosphate tetrasodium up-regulates the expression of PTEN, TGFBR2 and CDKN1C apoptosis and selective tumor suppressor genes[1].

Reference

[1]. Nidhi Jariwala, et al. Oncogenic Role of SND1 in Development and Progression of Hepatocellular Carcinoma. Cancer Res. 2017 Jun 15;77(12):3306-3316.
 [Content Brief]

[2]. Cohen J S, et al. Proton magnetic resonance studies of the tyrosine residues of staphylococcal nuclease using [3, 5-2H2] tyrosine[J]. Biochimica et Biophysica Acta (BBA)-Protein Structure, 1971, 236(2): 468-478.