For research use only. Not for therapeutic Use.
TMA-DPH is a hydrophobic fluorescent membrane probe (Ex=355 nm; Em=430 nm). TMA-DPH is able to anchor on the cell surface and localize to different regions of the phospholipid bilayer. By analyzing the fluorescence polarization values of TMA-DPH in the plasma membrane and membrane substructures, the fluidity of the cell membrane can be determined[1][2][3].
Upon contact with cells, TMA-DPH is immediately integrated into the plasma membrane and subsequently concentrated in lysosomes and highly acidic late endosomes[1].
The TMA-DPH fluorescence lifetime, which remained constant at concentrations below 2 μM, was 6.2 ± 0.2 ns. When the concentration is higher than 2 μM, there is a significant decrease with increasing concentration; when the concentration exceeds 5 μM, the rate of decrease decreases. TMA-DPH fluorescence anisotropy shows a similar evolution to the fluorescence lifetime. First, it remains constant at 0.283±0.003 at concentrations below 2 μM. There is a significant decrease with increasing concentration when the concentration is higher than 2 μM; then it rapidly decreases to 0.270±0.003 at 5 μM, and the subsequent decrease rate decreases significantly[2].
| Catalog Number | I012978 |
| CAS Number | 115534-33-3 |
| Synonyms | 4-methylbenzenesulfonate;trimethyl-[4-[(1E,3E,5E)-6-phenylhexa-1,3,5-trienyl]phenyl]azanium |
| Molecular Formula | C28H31NO3S |
| Purity | ≥95% |
| InChI | InChI=1S/C21H24N.C7H8O3S/c1-22(2,3)21-17-15-20(16-18-21)14-8-5-4-7-11-19-12-9-6-10-13-19;1-6-2-4-7(5-3-6)11(8,9)10/h4-18H,1-3H3;2-5H,1H3,(H,8,9,10)/q+1;/p-1/b5-4+,11-7+,14-8+; |
| InChIKey | ZKARERKEBVSZCX-VMDDUYISSA-M |
| SMILES | CC1=CC=C(C=C1)S(=O)(=O)[O-].C[N+](C)(C)C1=CC=C(C=C1)C=CC=CC=CC2=CC=CC=C2 |
| Reference | [1]. Illinger D, et al. The kinetic aspects of intracellular fluorescence labeling with TMA-DPH support the maturation model for endocytosis in L929 cells. J Cell Biol. 1994 May;125(4):783-94. [2]. Illinger D, et al. A comparison of the fluorescence properties of TMA-DPH as a probe for plasma membrane and for endocytic membrane. Biochim Biophys Acta. 1995 Oct 4;1239(1):58-66. [3]. Benedetti A, et al. Plasma membrane fluidity in isolated rat hepatocytes: comparative study using DPH and TMA-DPH as fluorescent probes. J Gastroenterol Hepatol. 1989 May-Jun;4(3):221-7. |
