TOOS

• CAT Number: I013901
• CAS Number: 82692-93-1
• Molecular Formula: C12H18NNaO4S
• Molecular Weight: 295.33
• Purity: ≥95%
• Synonyms: sodium;3-(N-ethyl-3-methylanilino)-2-hydroxypropane-1-sulfonate
• InChI: InChI=1S/C12H19NO4S.Na/c1-3-13(8-12(14)9-18(15,16)17)11-6-4-5-10(2)7-11;/h4-7,12,14H,3,8-9H2,1-2H3,(H,15,16,17);/q;+1/p-1
• InChIKey: IRQRBVOQGUPTLG-UHFFFAOYSA-M
• SMILES: CCN(CC(CS(=O)(=O)[O-])O)C1=CC=CC(=C1)C.[Na+]

TOOS (TOOS sodium salt) is a highly water-soluble aniline derivative widely used in diagnostics and biological experiments. TOOS can be combined with 3-methyl-2-benzothiazolinone hydrazone hydrochloride (MBTH) to form a chromogenic system to measure oxidase activity. In the MBTH-TOOS chromogenic system, MBTH is catalytically oxidized to produce (-NH) free radicals, which react with TOOS to form colorless compounds. Furthermore, the colorless compound undergoes a disproportionation reaction to produce a blue-violet quinoid compound[1].
MBTH-TOOS chromogenic system[1] Cit-PtNPs oxidase as an example: Mix 50 μL Cit-PtNP, 250 μL 3 mM TOOS, and 200 μL 0.5 mM MBTH into 500 μL Tris-HCl buffer solution (10 mM, pH=8.0) and incubate at 40°C for 25 min. Subsequently, the absorbance was measured at 590 nm and the oxidase activity level was calculated.

Reference

[1]. Lin X, et al. Boosting the oxidase-like activity of platinum nanozyme in MBTH-TOOS chromogenic system for detection of trypsin and its inhibitor. Talanta. 2021 Nov 1;234:122647.
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