TRFS-red

• CAT Number: I037477
• Molecular Formula: C28H28ClN3O11S2
• Molecular Weight: 682.12
• Purity: ≥95%
• Synonyms: [5-(dithiolan-4-yloxycarbonylamino)benzo[a]phenoxazin-9-ylidene]-bis(3-methoxy-3-oxopropyl)azanium;perchlorate
• InChI: InChI=1S/C28H27N3O7S2.ClHO4/c1-35-25(32)9-11-31(12-10-26(33)36-2)17-7-8-21-23(13-17)38-24-14-22(30-28(34)37-18-15-39-40-16-18)19-5-3-4-6-20(19)27(24)29-21;2-1(3,4)5/h3-8,13-14,18H,9-12,15-16H2,1-2H3;(H,2,3,4,5)
• InChIKey: SGZAVMKJSBHPCL-UHFFFAOYSA-N
• SMILES: COC(=O)CC[N+](=C1C=CC2=NC3=C(C=C(C4=CC=CC=C43)NC(=O)OC5CSSC5)OC2=C1)CCC(=O)OC.[O-]Cl(=O)(=O)=O

TRFS-red, a red fluorescence emission off-on probe, is selective for thioredoxin reductase (TrxR). TRFS-red exhibits high response rate and sensitivity. TRFS-red can be used for imaging live cells[1].
TRFS-red (1 μM; 30-120 min) gives red fluorescence signal in live HeLa cells, and the red fluorescence is predominantly distributed in the cytosol[1].
TRFS-red (10 μM) has a maximal absorption at ~530 nm in TE bu?er (50 mM Tris-HCl and 1 mM EDTA, pH 7.4)[1].
TRFS-red displays a reliable fluorescence signal in the pH range of 5.5-8.5[1].

Reference

[1]. Ma H, et, al. A fast response and red emission probe for mammalian thioredoxin reductase. Chem Commun (Camb). 2016 Oct 4;52(81):12060-12063.
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