Tyrphostin A1

• CAT Number: I002958
• CAS Number: 2826-26-8
• Molecular Formula: C11H8N2O
• Molecular Weight: 184.19
• Purity: ≥95%
• Target: Interleukin Related
• Solubility: ethanol：25 mg/ml ；DMSO：25 mg/ml； chloroform：50 mg/ml ；
• Storage: Store at -20°C

Tyrphostin A1(AG9) inhibits CD40L-stimulated IL-12 production in macrophage cultures and antigen-induced generation of Th1 cells.IC50 value:Target: IL-12 production inhibitorAddition of increasing concentration of A1 resulted in a dose dependent decrease of IL-12 p40, with maximal inhibition (62.5%) occurring at a dose of 10 μM. tyrphostin A1 blocks CD40L-induced translocation of NF-κB to the nucleus, and reduces the activation of IL-12 p40 gene. In vivo therapy with A1 leads to decrease in generation of myelin basic protein (MBP) specific encephalitogenic T cells. In addition, treatment of SJL/J mice with A1 results in attenuation of experimental allergic encephalomyelitis (EAE) [1]. Tyrphostin A1 is a much weaker inhibitor of TK than other tyrphostins (IC50 ?1250 ?M for epidermal growth factor receptor (EGFR) kinase: Ref. 1), and therefore often used to differentiate TK-mediated effects of tyrphostins from other non-specific effects [2].

Reference

[1]. Du C, et al. Inhibition of CD40 signaling pathway by tyrphostin A1 reduces secretion of IL-12 in macrophage, Th1 cell development and experimental allergic encephalomyelitis in SJL/J mice. J Neuroimmunol. 2001 Mar 1;114(1-2):69-79.
[2]. Ogura T, et al. Activation of background membrane conductance by the tyrosine kinase inhibitor tyrphostin A23 and its inactive analog tyrphostin A1 in guinea pig ventricular myocytes. Jpn J Pharmacol. 2001 Nov;87(3):235-9.