U-73343

• CAT Number: I009921
• CAS Number: 142878-12-4
• Molecular Formula: C29H42N2O3
• Molecular Weight: 466.67
• Purity: ≥95%
• Target: Phospholipase
• Solubility: Soluble in DMSO, not in water
• Storage: Store at RT
• IUPAC Name: 1-[6-[[(8R,9S,13S,14S,17S)-3-methoxy-13-methyl-6,7,8,9,11,12,14,15,16,17-decahydrocyclopenta[a]phenanthren-17-yl]amino]hexyl]pyrrolidine-2,5-dione
• InChI: InChI=1S/C29H42N2O3/c1-29-16-15-23-22-10-8-21(34-2)19-20(22)7-9-24(23)25(29)11-12-26(29)30-17-5-3-4-6-18-31-27(32)13-14-28(31)33/h8,10,19,23-26,30H,3-7,9,11-18H2,1-2H3/t23-,24-,25+,26+,29+/m1/s1
• InChIKey: CJHWFIUASFBCKN-ZRJUGLEFSA-N
• SMILES: CC12CCC3C(C1CCC2NCCCCCCN4C(=O)CCC4=O)CCC5=C3C=CC(=C5)OC

U-73343 (Cat No.: I009921) is a selective phospholipase C (PLC) inhibitor that lacks antioxidant properties, distinguishing it from its structural analog U-73122. It is commonly used in biochemical and pharmacological research to study PLC-mediated signal transduction pathways, particularly in cell signaling and calcium mobilization. Unlike U-73122, U-73343 exhibits significantly reduced inhibitory activity, making it useful as a negative control in experiments investigating PLC-dependent processes. Its applications are mainly in cancer, inflammation, and neurological disease research to elucidate PLC-related cellular mechanisms.

Reference

1: Takenouchi T, Ogihara K, Sato M, Kitani H. Inhibitory effects of U73122 and U73343 on Ca2+ influx and pore formation induced by the activation of P2X7 nucleotide receptors in mouse microglial cell line. Biochim Biophys Acta. 2005 Nov 15;1726(2):177-86. Epub 2005 Aug 15. PubMed PMID: 16122875.2:Bosch RR, Patel AM, Van Emst-de Vries SE, Smeets RL, De Pont JJ, Willems PH. U73122 and U73343 inhibit receptor-mediated phospholipase D activation downstream of phospholipase C in CHO cells. Eur J Pharmacol. 1998 Apr 10;346(2-3):345-51. PubMed PMID: 9652379.3:Muto Y, Nagao T, Urushidani T. The putative phospholipase C inhibitor U73122 and its negative control, U73343, elicit unexpected effects on the rabbit parietal cell. J Pharmacol Exp Ther. 1997 Sep;282(3):1379-88. PubMed PMID: 9316850.4:Heemskerk JW, Feijge MA, Sage SO, Farndale RW. Human platelet activation is inhibited upstream of the activation of phospholipase A2 by U73343. Biochem Pharmacol. 1997 May 9;53(9):1257-62. PubMed PMID: 9214686.