WST-8

• CAT Number: R052627
• CAS Number: 193149-74-5
• Molecular Formula: C20H13N6O11S2 • Na
• Molecular Weight: 600.5
• Purity: ≥95%
• Synonyms: 5-(2,4-Disulfophenyl)-3-(2-methoxy-4-nitrophenyl)-2-(4-nitrophenyl)-2H-tetrazolium Inner Salt Sodium Salt
• Storage: -20°C
• InChIKey: VSIVTUIKYVGDCX-UHFFFAOYSA-M

WST-8 is a water-soluble tetrazolium salt used for assessing cell metabolic activity. At neutral pH and in the presence of the intermediate electron acceptor, 1-methoxy phenazine methosulfate, NADPH-dependent cellular oxidoreductases, acting via plasma membrane electron transport, reduce the cell-impermeant WST-8 outside the cell to a water-soluble formazan dye with absorbance max at 460 nm. It is typically used as a cell viability indicator in cell proliferation assays.