YM-1

• CAT Number: I041688
• CAS Number: 409086-68-6
• Molecular Formula: C20H20ClN3OS2
• Molecular Weight: 417.98
• Purity: ≥95%
• Synonyms: (2Z,5E)-3-ethyl-5-(3-methyl-1,3-benzothiazol-2-ylidene)-2-[(1-methylpyridin-1-ium-2-yl)methylidene]-1,3-thiazolidin-4-one;chloride
• InChI: InChI=1S/C20H20N3OS2.ClH/c1-4-23-17(13-14-9-7-8-12-21(14)2)26-18(19(23)24)20-22(3)15-10-5-6-11-16(15)25-20;/h5-13H,4H2,1-3H3;1H/q+1;/p-1/b20-18+
• InChIKey: KEQDCNWQIQWINZ-KPJFUTMLSA-M
• SMILES: CCN1C(=CC2=CC=CC=[N+]2C)SC(=C3N(C4=CC=CC=C4S3)C)C1=O.[Cl-]

YM-1 is a stable and soluble MKT-077 (HY-15096) analog and an orally active Hsp70 inhibitor. YM-1 induces cell death of HeLa cells and up-regulates the level of p53 and p21 proteins[1][2].
YM-1 promotes Hsp70-dependent steps in nNOS maturation and partially blocks formation of the ATP-bound form[1].
YM-1 (0-200 μM) activates the binding of Hsp70 to its unfolded substrate[1].
YM-1 (0.001-1000 μM) converts Hsp70 to its tight-affinity conformation and shows binding efficacy to Hsp70 with an IC50 value of 8.2 μM[1].
YM-1 (0, 0.1, 0.5 and 1 μM; 24 hours) promotes nNOS ubiquitination[1].
YM-1 (5 and 10 μM; 24 and 48 hours) induces cell death of HeLa cells and growth arrest of hTERT-RPE1 cells[2].
YM-1 (10 μM; 48 hours) up-regulates p53 and p21 proteins and down-regulates FoxM1 and survivin[2].
YM-1 (1 mM; oral administration, for 7 days) rescues polyQ toxicity in Drosophila by activating Hsp70[1].

Reference

[1]. Wang AM, et al. Activation of Hsp70 reduces neurotoxicity by promoting polyglutamine protein degradation. Nat Chem Biol. 2013 Feb;9(2):112-8.
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[2]. Khondoker Md Zulfiker Rahman, et al. Effect of an Inhibitor of HSP70, YM-1, on Hikeshi Knockout Cells. Thermal Medicine. 2017, 33(4):129-134.