Z-Arg-AMC hydrochloride

• CAT Number: I029773
• CAS Number: 70375-22-3
• Molecular Formula: C24H28ClN5O5
• Molecular Weight: 501.96
• Purity: ≥95%
• Synonyms: benzyl N-[(2S)-5-(diaminomethylideneamino)-1-[(4-methyl-2-oxochromen-7-yl)amino]-1-oxopentan-2-yl]carbamate;hydrochloride
• InChI: InChI=1S/C24H27N5O5.ClH/c1-15-12-21(30)34-20-13-17(9-10-18(15)20)28-22(31)19(8-5-11-27-23(25)26)29-24(32)33-14-16-6-3-2-4-7-16;/h2-4,6-7,9-10,12-13,19H,5,8,11,14H2,1H3,(H,28,31)(H,29,32)(H4,25,26,27);1H/t19-;/m0./s1
• InChIKey: JYWCRDUTYUBXOL-FYZYNONXSA-N
• SMILES: CC1=CC(=O)OC2=C1C=CC(=C2)NC(=O)[C@H](CCCN=C(N)N)NC(=O)OCC3=CC=CC=C3.Cl

Z-Arg-AMC hydrochloride (Cat No.:I029773) is a fluorogenic substrate used in enzymatic assays to measure protease activity, particularly arginine-specific enzymes such as trypsin and certain caspases. Upon cleavage by the target protease, the AMC (7-amido-4-methylcoumarin) moiety is released, producing a measurable fluorescent signal. The Z (benzyloxycarbonyl) group protects the N-terminus, enhancing substrate stability. This compound is widely used in biochemical and pharmaceutical research to study enzyme kinetics, screen for inhibitors, and monitor protease-related pathways involved in inflammation, apoptosis, and cancer progression. The hydrochloride form improves its solubility and handling.