Z-FR-AMC TFA

• CAT Number: I038689
• Molecular Formula: C35H37F3N6O8
• Molecular Weight: 726.71
• Purity: 98%
• Synonyms: Z-FR-AMC TFA; ZFRAMC TFA; Z FR AMC TFA; Z-FRAMC TFA; Z-FR AMC TFA; ZFR-AMC TFA; Z FR-AMC TFA; Z-FR-7-amino-4-Methylcoumarin; Z-Phe-Arg-AMC
• Solubility: To be determined
• Appearance: Solid powder
• Storage: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
• IUPAC Name: benzyl N-[(2S)-1-[[(2S)-5-(diaminomethylideneamino)-1-[(4-methyl-2-oxochromen-7-yl)amino]-1-oxopentan-2-yl]amino]-1-oxo-3-phenylpropan-2-yl]carbamate;2,2,2-trifluoroacetic acid
• InChI: InChI=1S/C33H36N6O6.C2HF3O2/c1-21-17-29(40)45-28-19-24(14-15-25(21)28)37-30(41)26(13-8-16-36-32(34)35)38-31(42)27(18-22-9-4-2-5-10-22)39-33(43)44-20-23-11-6-3-7-12-23;3-2(4,5)1(6)7/h2-7,9-12,14-15,17,19,26-27H,8,13,16,18,20H2,1H3,(H,37,41)(H,38,42)(H,39,43)(H4,34,35,36);(H,6,7)/t26-,27-;/m0./s1
• InChIKey: AVPSADNFAQBJBZ-WMXJXTQLSA-N
• SMILES: CC1=CC(=O)OC2=C1C=CC(=C2)NC(=O)[C@H](CCCN=C(N)N)NC(=O)[C@H](CC3=CC=CC=C3)NC(=O)OCC4=CC=CC=C4.C(=O)(C(F)(F)F)O

Z-FR-AMC TFA (Cat No.:I038689) is a synthetic peptide substrate used primarily in biochemical assays to measure protease activity. It consists of a substrate linked to a fluorogenic molecule, AMC (7-amino-4-methylcoumarin), which fluoresces when cleaved by specific proteases, such as caspases or cathepsins. The “Z” represents a benzyloxycarbonyl protecting group, while “FR” is a peptide sequence that is cleaved by target enzymes. Z-FR-AMC TFA is used in research to study enzyme kinetics, protease inhibition, and cellular processes involving protein degradation, providing insights into disease mechanisms and therapeutic targets.

Reference

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