Z-Gly-Gly-Arg-AMC

• CAT Number: I013794
• CAS Number: 66216-78-2
• Molecular Formula: C₂₈H₃₃N₇O₇
• Molecular Weight: 693.61
• Purity: ≥95%
• Target: PeptidesDye Reagents
• Solubility: DMSO
• IUPAC Name: benzyl N-[2-[[2-[[(2S)-5-(diaminomethylideneamino)-1-[(4-methyl-2-oxochromen-7-yl)amino]-1-oxopentan-2-yl]amino]-2-oxoethyl]amino]-2-oxoethyl]carbamate
• InChI: InChI=1S/C28H33N7O7/c1-17-12-25(38)42-22-13-19(9-10-20(17)22)34-26(39)21(8-5-11-31-27(29)30)35-24(37)15-32-23(36)14-33-28(40)41-16-18-6-3-2-4-7-18/h2-4,6-7,9-10,12-13,21H,5,8,11,14-16H2,1H3,(H,32,36)(H,33,40)(H,34,39)(H,35,37)(H4,29,30,31)/t21-/m0/s1
• InChIKey: SXTGIAYWYXVNLT-NRFANRHFSA-N
• SMILES: CC1=CC(=O)OC2=C1C=CC(=C2)NC(=O)C(CCCN=C(N)N)NC(=O)CNC(=O)CNC(=O)OCC3=CC=CC=C3

Z-Gly-Gly-Arg-AMC(CAT: I013794)) is a fluorogenic peptide substrate commonly used in enzymatic assays to study serine proteases, such as thrombin and trypsin. Upon enzymatic cleavage of the arginine-AMC bond, the fluorescent group 7-amido-4-methylcoumarin (AMC) is released, emitting fluorescence measurable by spectrofluorometry. Z-Gly-Gly-Arg-AMC is widely employed in research to evaluate protease activity, inhibitor screening, and kinetic studies. Its sensitivity and reliability make it a valuable tool in biochemical and pharmaceutical research for understanding protease-related mechanisms and developing therapeutic agents.