(Z)-Mirin

• CAT Number: I000727
• CAS Number: 1198097-97-0
• Molecular Formula: C10H8N2O2S
• Molecular Weight: 220.25
• Purity: ≥95%

(Z)-Mirin is an MRN (Mre11-Rad50-Nbs1) inhibitor. (Z)-Mirin prevents MRN-dependent activation of ATM without affecting ATM protein kinase activity. (Z)-Mirin inhibits Mre11-associated exonuclease activity. (Z)-Mirin increases apoptosis, triggers a G2/M checkpoint and strongly inhibits homology-directed repair (HDR)[1][2].
(Z)-Mirin (50, 100, 500 μM) inhibits ATM-dependent phosphorylation of Nbs1 and Chk2 and the MRN-dependent autophosphorylation of ATM at Ser1981 in response to DNA double-strand breaks (DSBs). (Z)-Mirin (100 μM) inhibits Mre11 nuclease activity[1].
(Z)-Mirin (10-100 μM; 24 h) shows 50% cytotoxicity at 50 μM in HEK293 cells[1].
(Z)-Mirin (10-100 μM) induces a substantial G2 arrest at concentrations of 50 μM and 100 μM[1].
(Z)-Mirin (18 µM; 48 h) increases apoptosis in PEO4 cells[2].

Reference

[1]. Rozier L, et al. The MRN-CtIP pathway is required for metaphase chromosome alignment. Mol Cell. 2013 Mar 28;49(6):1097-107.
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[2]. Lee JH, et al. Ataxia telangiectasia-mutated (ATM) kinase activity is regulated by ATP-driven conformational changes in the Mre11/Rad50/Nbs1 (MRN) complex. J Biol Chem. 2013 May 3;288(18):12840-51.
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[3]. Garner KM, et al. Corrected structure of Mirin, a small-molecule inhibitor of the Mre11-Rad50-Nbs1 complex. Nat Chem Biol. 2009 Mar;5(3):129-30.
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[4]. Aude Dupré, et al. A forward chemical genetic screen reveals an inhibitor of the Mre11-Rad50-Nbs1 complex. Nat Chem Biol. 2008 Feb;4(2):119-25.
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[5]. Adel Alblihy, et al. Selective Killing of BRCA2-Deficient Ovarian Cancer Cells via MRE11 Blockade. Int J Mol Sci. 2023 Jun 30;24(13):10966.
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