Z-Vad-fmk, non-methylated

• CAT Number: M132012
• CAS Number: 161401-82-7
• Molecular Formula: C21H28FN3O7
• Molecular Weight: 453.47
• Purity: ≥95%
• Synonyms: Z-Val-Ala-Asp-(OH)-Fluoromethyl Ketone
• Target: Caspase
• Storage: -20°C
• IUPAC Name: (3S)-5-fluoro-3-[[(2S)-2-[[(2S)-3-methyl-2-(phenylmethoxycarbonylamino)butanoyl]amino]propanoyl]amino]-4-oxopentanoic acid
• InChI: InChI=1S/C21H28FN3O7/c1-12(2)18(25-21(31)32-11-14-7-5-4-6-8-14)20(30)23-13(3)19(29)24-15(9-17(27)28)16(26)10-22/h4-8,12-13,15,18H,9-11H2,1-3H3,(H,23,30)(H,24,29)(H,25,31)(H,27,28)/t13-,15-,18-/m0/s1
• InChIKey: SUUHZYLYARUNIA-YEWWUXTCSA-N
• SMILES: C[C@@H](C(=O)N[C@@H](CC(=O)O)C(=O)CF)NC(=O)[C@H](C(C)C)NC(=O)OCC1=CC=CC=C1

Z-VAD-FMK (benzyloxycarbonyl-Val-Ala-Asp-fluoromethylketone)（Cat No.:M132012）is a potent, broad-spectrum inhibitor of caspases, the enzymes responsible for executing apoptosis. It is commonly used in research to study apoptosis pathways by blocking the activation of caspases, thus preventing programmed cell death. The “Z” denotes the benzyloxycarbonyl group, and the “FMK” refers to the fluoromethylketone, which covalently binds to the active site of caspases. Non-methylated Z-VAD-FMK is simply a variant without a methyl group at certain positions in the molecule, which might influence its binding affinity or stability. It is often used in cell culture experiments to investigate the role of caspases in various diseases.