Recombinant Beta-lactamase SHV-1 (bla)

• Purity: >85% (SDS-PAGE)
• Host Species: Escherichia coli
• Accession: P0AD63
• Gene: bla
• CAT.NO: B000296
• Expression Systems: E.coli In Vivo Biotinylation in E.coli Yeast Baculovirus Mammalian cell
• Product Name: Recombinant Beta-lactamase SHV-1 (bla)
• Source: E.coli;In Vivo Biotinylation in E.coli;Yeast;Baculovirus;Mammalian cell
• Protein Expression Range: 22-286
• Tag: Tag type will be determined during the manufacturing process.
• Form: Lyophilized powder
• Buffer: Tris/PBS-based buffer, 6% Trehalose.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Alternative Names: bla; shv1Beta-lactamase SHV-1; EC 3.5.2.6; PIT-2
• Target Protein Sequence: SPQPLEQIK LSESQLSGRV GMIEMDLASG RTLTAWRADE RFPMMSTFKV VLCGAVLARV DAGDEQLERK IHYRQQDLVD YSPVSEKHLA DGMTVGELCA AAITMSDNSA ANLLLATVGG PAGLTAFLRQ IGDNVTRLDR WETELNEALP GDARDTTTPA SMAATLRKLL TSQRLSARSQ RQLLQWMVDD RVAGPLIRSV LPAGWFIADK TGAGERGARG IVALLGPNNK AERIVVIYLR DTPASMAERN QQIAGIGAAL IEHWQR
• Protein Length: Full Length of Mature Protein
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
• Shelf Life: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.; Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Recombinant Beta-lactamase SHV-1 (bla) is an enzyme that provides bacterial resistance to beta-lactam antibiotics, particularly penicillins and some cephalosporins. It works by hydrolyzing the beta-lactam ring, rendering the antibiotic ineffective. SHV-1 is commonly found in Escherichia coli and other enteric bacteria, often contributing to antibiotic resistance in clinical settings. The recombinant form of this enzyme is used in laboratory settings for research purposes, including studying antibiotic resistance mechanisms. SHV-1 does not typically confer resistance to carbapenems, but it can provide broad-spectrum resistance to many beta-lactam antibiotics.