Recombinant Chicken Tyrosine-protein kinase BTK (BTK), partial

• Purity: >85% (SDS-PAGE)
• Host Species: Gallus gallus (Chicken)
• Accession: Q8JH64
• Gene: BTK
• CAT.NO: B000146
• Expression Systems: E.coli In Vivo Biotinylation in E.coli Yeast Baculovirus Mammalian cell
• Product Name: Recombinant Chicken Tyrosine-protein kinase BTK (BTK), partial
• Source: E.coli;In Vivo Biotinylation in E.coli;Yeast;Baculovirus;Mammalian cell
• Tag: Tag type will be determined during the manufacturing process.
• Form: Lyophilized powder
• Buffer: Tris/PBS-based buffer, 6% Trehalose.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Alternative Names: BTKTyrosine-protein kinase BTK; EC 2.7.10.2; Bruton tyrosine kinase
• Protein Length: Partial
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
• Shelf Life: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.; Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

This non-receptor tyrosine kinase is essential for B lymphocyte development, differentiation, and signaling. When an antigen binds to the B-cell antigen receptor (BCR), it triggers signaling pathways that lead to B-cell activation. Following BCR engagement at the plasma membrane, the kinase phosphorylates PLCG2 at multiple sites, initiating a downstream signaling cascade. This cascade involves calcium mobilization and subsequent activation of protein kinase C (PKC) family members. The phosphorylation of PLCG2 occurs in close cooperation with the adapter protein B-cell linker protein (BLNK), which facilitates the signaling process necessary for effective B-cell function.