In the E. coli 70S ribosome during the initiation state, protein S19 is modeled to contact the 23S rRNA of the 50S subunit, forming part of bridge B1a. This bridge is disrupted in models with bound EF-G, and its 23S rRNA contact site alternates between S13 and S19 depending on the ribosomal state. High-resolution ribosome structures reveal dynamic interactions in bridge B1b involving L5, S13, and S19, while bridge B1a is unresolved in crystallized ribosomes due to 23S rRNA disorder.
S19 forms a complex with S13, which binds tightly to 16S rRNA. It also interacts with the A-site tRNA, playing a critical role in ribosomal dynamics and tRNA positioning during translation. These interactions highlight its importance in ribosome structure and function.
| Product Name | Recombinant E.Coli 30S Ribosomal Protein S19 (RPSS) Protein (His) |
| Accession | P0A7U3 |
| Purity | Greater than 90% as determined by SDS-PAGE. |
| Host Species | E.coli |
| Gene | RPSS |
| Source | E.coli |
| Protein Expression Range | 2-92aa |
| Tag | N-6His |
| Molecular Mass | 14.3kDa |
| Form | Liquid or Lyophilized powder |
| Buffer | Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
| Storage | 1. Store at -20¡«C/-80¡«C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4¡«C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20¡«C/-80¡«C. Protein in lyophilized powder form is stable for up to 12 months at -20¡«C/-80¡«C. |
