Protein S4 plays a pivotal role in translational accuracy alongside S5 and S12. It is associated with suppressors of streptomycin-dependent mutations in protein S12, some of which reduce translational fidelity (ribosomal ambiguity mutations, or ram). Additionally, S4 may contribute to mRNA unwinding during translation by forming part of a processivity clamp.
As a translational repressor, S4 regulates the alpha-operon, which encodes S13, S11, S4, RNA polymerase alpha subunit, and L17, by binding to its mRNA. It also acts as a rho-dependent antiterminator, increasing rRNA synthesis when excess protein is present, thereby accelerating homeostasis restoration. S4 directly interacts with RNA polymerase and is a component of the processive rRNA transcription and antitermination complex (rrnTAC). This complex forms a chaperone-like ring around the RNA exit tunnel of RNA polymerase, facilitating rapid rRNA transcription, antitermination, and cotranscriptional rRNA folding. S4 is particularly involved in long-distance rRNA annealing required for accurate pre-rRNA processing and ribosome biogenesis.
| Product Name | Recombinant E.Coli 30S Ribosomal Protein S4 (RPSD) Protein (His) |
| Accession | P0A7V8 |
| Purity | Greater than 90% as determined by SDS-PAGE. |
| Host Species | E.coli |
| Gene | RPSD |
| Source | E.coli |
| Protein Expression Range | 2-206aa |
| Tag | N-6His |
| Molecular Mass | 27.3kDa |
| Form | Liquid or Lyophilized powder |
| Buffer | Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
| Storage | 1. Store at -20¡«C/-80¡«C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4¡«C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20¡«C/-80¡«C. Protein in lyophilized powder form is stable for up to 12 months at -20¡«C/-80¡«C. |
