Recombinant Extended-spectrum beta-lactamase PER-1 (per1)

• Purity: >85% (SDS-PAGE)
• Host Species: Pseudomonas aeruginosa
• Accession: P37321
• Gene: per1
• CAT.NO: B000238
• Expression Systems: E.coli In Vivo Biotinylation in E.coli Yeast Baculovirus Mammalian cell
• Product Name: Recombinant Extended-spectrum beta-lactamase PER-1 (per1)
• Source: E.coli;In Vivo Biotinylation in E.coli;Yeast;Baculovirus;Mammalian cell
• Protein Expression Range: 27-308
• Tag: Tag type will be determined during the manufacturing process.
• Form: Lyophilized powder
• Buffer: Tris/PBS-based buffer, 6% Trehalose.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Target Protein Sequence: QSPL LKEQIESIVI GKKATVGVAV WGPDDLEPLL INPFEKFPMQ SVFKLHLAML VLHQVDQGKL DLNQTVIVNR AKVLQNTWAP IMKAYQGDEF SVPVQQLLQY SVSHSDNVAC DLLFELVGGP AALHDYIQSM GIKETAVVAN EAQMHADDQV QYQNWTSMKG AAEILKKFEQ KTQLSETSQA LLWKWMVETT TGPERLKGLL PAGTVVAHKT GTSGIKAGKT AATNDLGIIL LPDGRPLLVA VFVKDSAESS RTNEAIIAQV AQTAYQFELK KLSALSPN
• Protein Length: Full Length of Mature Protein
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
• Shelf Life: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature; and the stability of the protein itself.; Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized; form is 12 months at -20°C/-80°C.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

This extended-spectrum beta-lactamase (ESBL) provides resistance to penicillins and first-, second-, and third-generation cephalosporins, but does not affect carbapenems, such as imipenem, in the JM109 strain of E. coli. The enzyme exhibits cefotaxime-hydrolyzing activity, enabling the bacteria to break down this particular cephalosporin and resist its antibacterial effects. This resistance mechanism allows the bacterium to survive in the presence of common beta-lactam antibiotics, contributing to the challenge of treating infections caused by ESBL-producing E. coli strains. The enzyme’s activity is critical for its role in antimicrobial resistance.