Recombinant Human 5-hydroxytryptamine receptor 2A (HTR2A)

• Host Species: Homo sapiens (Human)
• Accession: P28223
• CAT.NO: B000171
• Expression Systems: In vitro E.coli expression system
• Product Name: Recombinant Human 5-hydroxytryptamine receptor 2A (HTR2A)
• Protein Expression Range: 1-471
• Tag: Tag type will be determined during the manufacturing process.
• Form: Lyophilized powder
• Buffer: Tris/PBS-based buffer, 6% Trehalose.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Alternative Names: HTR2A; HTR2; 5-hydroxytryptamine receptor 2A; 5-HT-2; 5-HT-2A; Serotonin receptor 2A
• Target Protein Sequence: MDILCEENTSLSSTTNSLMQLNDDTRLYSNDFNSGEANTSDAFNWTVDSENRTNLSCEGCLSPSCLSLLHLQEKNWSALLTAVVIILTIAGNILVIMAVSLEKKLQNATNYFLMSLAIADMLLGFLVMPVSMLTILYGYRWPLPSKLCAVWIYLDVLFSTASIMHLCAISLDRYVAIQNPIHHSRFNSRTKAFLKIIAVWTISVGISMPIPVFGLQDDSKVFKEGSCLLADDNFVLIGSFVSFFIPLTIMVITYFLTIKSLQKEATLCVSDLGTRAKLASFSFLPQSSLSSEKLFQRSIHREPGSYTGRRTMQSISNEQKACKVLGIVFFLFVVMWCPFFITNIMAVICKESCNEDVIGALLNVFVWIGYLSSAVNPLVYTLFNKTYRSAFSRYIQCQYKENKKPLQLILVNTIPALAYKSSQLQMGQKKNSKQDAKTTDNDCSMVALGKQHSEEASKDNSDGVNEKVSCV
• Protein Length: full length protein
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
• Shelf Life: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.; Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

The G-protein coupled receptor for 5-hydroxytryptamine (serotonin), also serves as a receptor for several psychoactive substances, including mescaline, psilocybin, DOI, and LSD. Binding of ligands induces a conformational change, activating G proteins and modulating downstream effectors. HTR2A is coupled to G(q)/G11 G alpha proteins, which activate phospholipase C-beta. This results in the production of diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3), which respectively regulate phosphatidylinositol 3-kinase activity and promote the release of Ca2+ ions from intracellular stores, influencing various cellular processes.