Recombinant Human 5'-Nucleotidase (NT5E) Protein (His), Active

• Purity: Greater than 90% as determined by SDS-PAGE.
• Host Species: Homo sapiens (Human)
• Accession: P21589
• Gene: NT5E
• CAT.NO: B000271
• Expression Systems: Mammalian cell
• Product Name: Recombinant Human 5'-Nucleotidase (NT5E) Protein (His), Active
• Endotoxin: Less than 1.0 EU/μg as determined by LAL method.
• Protein Expression Range: 27-547aa
• Tag: C-6His
• Molecular Mass: 58.76 kDa
• Form: Liquid or Lyophilized powder
• Buffer: 0.2 μm Filtered 20 mM Tris-HCl, 120 mM NaCl, 4 mM CaCl2, 20% Glycerol, pH 7.5
• Storage: 1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use.; 2. Avoid repeated freeze-thaw cycles.; 3. Store working aliquots at 4°C for up to one week.; 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
• Bioactivity: Specific activity as determined by its ability to hydrolyze the 5’-phosphate group from the substrate adenosine-5’-monophosphate (AMP) is greater than 15000 pmol/min/ug. The orthophosphate product is measured by a malachite green phosphate detection kit.
• Research Area: Cardiovascular
• Protein Length: Partial

Recombinant Human 5′-Nucleotidase (NT5E) Protein (His), Active, is generated using our mammalian cell expression system. This active protein fragment is designed for research applications and is produced to ensure optimal functionality. The His-tagged version allows for easy purification and handling, making it suitable for various experimental needs. It plays a key role in nucleotide metabolism by hydrolyzing nucleotides into nucleosides, which is vital for regulating nucleotide concentrations in cells. This protein is ideal for studies related to cellular processes and enzymatic activity in a research setting.