Recombinant Human ALK tyrosine kinase receptor (ALK), partial

• Purity: >85% (SDS-PAGE)
• Host Species: Homo sapiens (Human)
• Accession: Q9UM73
• Gene: ALK
• CAT.NO: B000246
• Expression Systems: E.coli In Vivo Biotinylation in E.coli Yeast Baculovirus Mammalian cell
• Product Name: Recombinant Human ALK tyrosine kinase receptor (ALK), partial
• Source: E.coli;In Vivo Biotinylation in E.coli;Yeast;Baculovirus;Mammalian cell
• Tag: Tag type will be determined during the manufacturing process.
• Form: Lyophilized powder
• Buffer: Tris/PBS-based buffer, 6% Trehalose.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Protein Length: Partial
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
• Shelf Life: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature; and the stability of the protein itself.; Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized; form is 12 months at -20°C/-80°C.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

This neuronal receptor tyrosine kinase is transiently expressed in specific regions of the central and peripheral nervous systems, playing a crucial role in nervous system development and differentiation. It also acts as a key regulator of body weight resistance, particularly in hypothalamic neurons, where it controls energy expenditure by negatively regulating white adipose tissue lipolysis and sympathetic tone to fine-tune energy homeostasis. Upon activation by the ALKAL2 ligand, it transduces extracellular signals into intracellular responses, activating the mitogen-activated protein kinase (MAPK) pathway. Ligand binding induces tyrosine kinase activation, resulting in phosphorylation of CBL, FRS2, IRS1, SHC1, and MAPK1/ERK2, MAPK3/ERK1.