Steroid hormone receptors are ligand-activated transcription factors that regulate gene expression in eukaryotic cells, influencing cellular proliferation and differentiation in target tissues. Their transcriptional activity is modulated by interactions with coactivators and corepressors. For example, ZBTB7A recruits NCOR1 and NCOR2 to androgen response elements (ARE) on target genes, suppressing androgen receptor signaling and androgen-induced cell proliferation. Additionally, NR0B2 down-regulates transcription activation.
Receptor activation occurs through ligand binding, while modulation may also involve interactions with kinases such as HIPK3 and ZIPK/DAPK3, which activate the receptor without phosphorylation. These mechanisms ensure precise regulation of gene expression, contributing to hormone-responsive tissue development and function.
| Product Name | Recombinant Human Androgen Receptor (AR) Protein (His-SUMO&Myc) |
| Accession | P10275 |
| Purity | Greater than 90% as determined by SDS-PAGE. |
| Host Species | Human |
| Gene | AR |
| Source | E.coli |
| Protein Expression Range | 551-919aa |
| Tag | N-10His-SUMO&C-Myc |
| Molecular Mass | 62.4kDa |
| Form | Liquid or Lyophilized powder |
| Buffer | Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
| Storage | 1. Store at -20¡«C/-80¡«C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4¡«C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20¡«C/-80¡«C. Protein in lyophilized powder form is stable for up to 12 months at -20¡«C/-80¡«C. |
