This protein suppresses apoptosis across various cell types, including factor-dependent lymphohematopoietic and neural cells, by regulating mitochondrial membrane permeability. It functions in a feedback loop with caspases, inhibiting their activity by preventing cytochrome c release from mitochondria and/or binding to the apoptosis-activating factor APAF-1.
Additionally, it inhibits autophagy under non-starvation conditions by interacting with BECN1 and AMBRA1, thereby suppressing their autophagy-related functions. This dual role highlights its importance in maintaining cellular homeostasis by modulating both apoptosis and autophagy.
The protein may also reduce inflammation by impairing NLRP1-inflammasome activation, which subsequently inhibits CASP1 activation and IL1B release. Its multifaceted regulatory functions underscore its critical role in cell survival, immune response, and inflammatory control.
| Product Name | Recombinant Human Apoptosis Regulator Bcl-2 (BCL2) Protein (His) |
| Accession | P10415 |
| Purity | Greater than 85% as determined by SDS-PAGE. |
| Host Species | Human |
| Gene | BCL2 |
| Source | E.coli |
| Protein Expression Range | 2-211aa |
| Tag | C-10His |
| Molecular Mass | 25.2 kDa |
| Form | Liquid or Lyophilized powder |
| Buffer | Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
| Storage | 1. Store at -20¡«C/-80¡«C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4¡«C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20¡«C/-80¡«C. Protein in lyophilized powder form is stable for up to 12 months at -20¡«C/-80¡«C. |
