The aryl hydrocarbon receptor (AHR) is a ligand-activated transcription factor that enables cellular adaptation to environmental, dietary, microbiome-derived, and metabolic compounds. AHR plays critical roles in development, immunity, and cancer regulation. Upon ligand binding, it translocates to the nucleus, heterodimerizes with ARNT, and induces transcription by binding to xenobiotic response elements (XRE). This activation regulates various processes, including angiogenesis, hematopoiesis, drug and lipid metabolism, cell motility, and immune modulation.
AHR recognizes xenobiotics as ligands, initiating the expression of phase I and II xenobiotic-metabolizing enzyme genes, such as CYP1A1, mediating the detoxification of halogenated aromatic hydrocarbons. Beyond xenobiotics, natural ligands from plants, microbiota, and endogenous metabolites, particularly tryptophan (Trp) derivatives like indoles and kynurenic acid, serve as potent AHR agonists. These ligands modulate tumor immunity, promoting cancer cell motility and suppressing adaptive immune responses, positioning AHR as a negative regulator of anti-tumor immunity.
Additionally, AHR influences the circadian clock by inhibiting the expression of PER1, a core circadian gene. It represses transcriptional activation of PER1 by the CLOCK-BMAL1 heterodimer. The ARNT
complex binds to the dioxin response element (5′-TGCGTG-3′) in target gene promoters, driving transcription. AHR’s multifaceted regulatory roles underscore its importance in environmental sensing, metabolic adaptation, and pathophysiology, particularly in immunity and cancer.
| Product Name | Recombinant Human Aryl Hydrocarbon Receptor (AHR) Protein (His) |
| Accession | P35869 |
| Purity | Greater than 85% as determined by SDS-PAGE. |
| Host Species | Human |
| Gene | AHR |
| Source | E.coli |
| Protein Expression Range | 220-420aa |
| Tag | N-10His |
| Molecular Mass | 29.0 kDa |
| Form | Liquid or Lyophilized powder |
| Buffer | Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
| Storage | 1. Store at -20¡«C/-80¡«C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4¡«C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20¡«C/-80¡«C. Protein in lyophilized powder form is stable for up to 12 months at -20¡«C/-80¡«C. |
