Recombinant Human Atp-Dependent Translocase Abcb1 (ABCB1) Protein (His)

• Purity: Greater than 90% as determined by SDS-PAGE.
• Host Species: Human
• Accession: P08183
• Gene: ABCB1
• CAT.NO: B000081
• Expression Systems: E.coli
• Product Name: Recombinant Human Atp-Dependent Translocase Abcb1 (ABCB1) Protein (His)
• Protein Expression Range: 236-297aa
• Tag: N-6His
• Molecular Mass: 10.8kDa
• Form: Liquid or Lyophilized powder
• Buffer: Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
• Storage: 1. Store at -20¡«C/-80¡«C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4¡«C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20¡«C/-80¡«C. Protein in lyophilized powder form is stable for up to 12 months at -20¡«C/-80¡«C.

This protein facilitates the translocation of drugs and phospholipids across cellular membranes. It catalyzes the energy-dependent “flop” of phospholipids, moving them from the cytoplasmic to the exoplasmic leaflet of the apical membrane. Its primary role involves the translocation of phosphatidylcholine, phosphatidylethanolamine, beta-D-glucosylceramides, and sphingomyelins.
As an energy-dependent efflux pump, it is critical in reducing drug accumulation in multidrug-resistant cells, contributing to drug resistance by actively exporting compounds out of the cell. This dual role in lipid transport and drug efflux underscores its significance in maintaining membrane asymmetry and influencing pharmacological responses in multidrug resistance contexts.