Recombinant Human C-X-C Chemokine Receptor Type 4 (CXCR4) Protein (His), Active

• Host Species: Human
• Accession: P61073
• Gene: CXCR4
• CAT.NO: B000021
• Product Name: Recombinant Human C-X-C Chemokine Receptor Type 4 (CXCR4) Protein (His), Active
• Endotoxin: Less than 1.0 EU/ug as determined by LAL method.
• Source: Mammalian cell
• Protein Expression Range: 1-352aa
• Tag: C-10His
• Molecular Mass: 41.5 kDa
• Form: Liquid
• Buffer: PBS, pH 7.4, 6% trehalose
• Storage: 1. Store at -20～C/-80～C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4～C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20～C/-80～C. Protein in lyophilized powder form is stable for up to 12 months at -20～C/-80～C.
• Bioactivity: Measured by its binding ability in a functional ELISA. Immobilized Human CXCR4 at 10 μg/mL can bind Anti-CXCR4 recombinant antibody , the EC 50 is 101.7-253.6 ng/mL.

This receptor binds the C-X-C chemokine CXCL12/SDF-1, triggering intracellular calcium ion increases and MAPK1/MAPK3 activation while engaging the AKT signaling cascade. It regulates cell migration, including during wound healing, and acts as a receptor for extracellular ubiquitin, enhancing calcium ion influx and reducing cellular cAMP levels. The receptor binds bacterial lipopolysaccharide (LPS), mediating LPS-induced inflammatory responses such as TNF secretion by monocytes.
It plays key roles in hematopoiesis, cardiac ventricular septum formation, and gastrointestinal vascularization through endothelial cell remodeling. Additionally, it contributes to cerebellar development and hippocampal neuron survival in the CNS (by similarity). In microbial infections, it functions as a coreceptor for HIV-1 X4 isolates (with CD4) and as a primary receptor for certain HIV-2 isolates, facilitating Env-mediated viral fusion.