Recombinant Human C5A Anaphylatoxin Chemotactic Receptor 1 (C5AR1) Protein (His), Active

• Host Species: Human
• Accession: P21730
• Gene: C5AR1
• CAT.NO: B000053
• Expression Systems: Mammalian cell
• Product Name: Recombinant Human C5A Anaphylatoxin Chemotactic Receptor 1 (C5AR1) Protein (His), Active
• Endotoxin: Less than 1.0 EU/ug as determined by LAL method.
• Protein Expression Range: 1-350aa
• Tag: C-10His
• Molecular Mass: 41.1 kDa
• Form: Liquid
• Buffer: PBS, pH 7.4, 6% trehalose
• Storage: 1. Store at -20～C/-80～C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4～C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20～C/-80～C. Protein in lyophilized powder form is stable for up to 12 months at -20～C/-80～C.
• Bioactivity: Measured by its binding ability in a functional ELISA. Immobilized human C5AR1 at 10 μg/mL can bind Anti-C5AR1 recombinant antibody , the EC 50 is 1.239-3.760 ng/mL.

The receptor for the chemotactic and inflammatory peptide anaphylatoxin C5a mediates immune and inflammatory responses. It binds C5a at two key sites: a high-affinity site on the extracellular N-terminus and an activation site in the transmembrane region, triggering downstream signaling. Receptor activation induces chemotaxis, granule enzyme release, intracellular calcium mobilization, and superoxide anion production, essential for immune cell recruitment and response. This receptor plays a critical role in orchestrating the inflammatory cascade and innate immune defense, making it a significant target in conditions involving excessive inflammation or immune dysregulation.