Recombinant Human Cd40 Ligand (CD40LG), Active

• Purity: Greater than 95% as determined by SDS-PAGE.
• Host Species: Homo sapiens (Human)
• Accession: P29965
• Gene: CD40LG
• CAT.NO: B000273
• Expression Systems: E.coli
• Product Name: Recombinant Human Cd40 Ligand (CD40LG), Active
• Endotoxin: Less than 1.0 EU/μg as determined by LAL method.
• Protein Expression Range: 113-261aa
• Tag: Tag-Free
• Molecular Mass: 16.2 kDa
• Form: Liquid or Lyophilized powder
• Buffer: Lyophilized from a 0.2 μm filtered 20 mM PB, 200 mM NaCl, 0.1 mM EDTA, pH 7.0
• Storage: 1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use.; 2. Avoid repeated freeze-thaw cycles.; 3. Store working aliquots at 4°C for up to one week.; 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
• Bioactivity: Immobilized Human CD40-Fc at 5μg/ml (100 μl/well) can bind Human CD40L . The ED50 of Human CD40L is less than 10 ng/ml.
• Research Area: Immunology
• Protein Length: Partial

This cytokine acts as a ligand for CD40/TNFRSF5, providing crucial costimulation for T-cell proliferation and cytokine production. Cross-linking on T-cells generates a costimulatory signal that enhances IL-4 and IL-10 production, working alongside TCR/CD3 ligation and CD28 costimulation. It activates NF-kappa-B and kinases MAPK8 and PAK2 in T-cells, inducing tyrosine phosphorylation of CD28 isoform 3. This cytokine also mediates B-cell proliferation in the absence of co-stimulation and promotes IgE production in the presence of IL-4. Additionally, it plays a role in immunoglobulin class switching, contributing to adaptive immune responses.