The activation of C5 by a C5 convertase triggers the assembly of the late complement components (C5-C9) into the membrane attack complex (MAC), which mediates cell lysis. During this process, C5b transiently binds C6, forming the C5b-C6 complex that serves as the foundation for lytic complex assembly.
C5a, an anaphylatoxin derived from proteolytic cleavage of C5, plays a critical role in the inflammatory response. Upon binding to its receptor, C5AR1, it induces intracellular calcium release, smooth muscle contraction, increased vascular permeability, and histamine release from mast cells and basophilic leukocytes. These actions amplify the local inflammatory process.
Additionally, C5a functions as a potent chemokine, stimulating the locomotion of polymorphonuclear leukocytes (PMNs) and directing their migration to sites of inflammation. This dual role in promoting inflammation and recruiting immune cells highlights its importance in immune defense and inflammatory regulation.
| Product Name | Recombinant Human Complement C5 (C5) Protein (His) |
| Accession | P01031 |
| Purity | Greater than 90% as determined by SDS-PAGE. |
| Host Species | Human |
| Gene | C5 |
| Source | Mammalian cell |
| Protein Expression Range | 678-751aa |
| Tag | N-6His |
| Molecular Mass | 12.3kDa |
| Form | Liquid or Lyophilized powder |
| Buffer | Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
| Storage | 1. Store at -20¡«C/-80¡«C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4¡«C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20¡«C/-80¡«C. Protein in lyophilized powder form is stable for up to 12 months at -20¡«C/-80¡«C. |
