This serine/threonine kinase is a core component of cyclin D-CDK4 (DC) complexes, which regulate the G1/S phase transition in the cell cycle by phosphorylating members of the retinoblastoma (RB) protein family, including RB1. Phosphorylation of RB1 disrupts RB/E2F complexes, releasing the transcription factor E2F to activate genes essential for G1 phase progression. RB1 is hypophosphorylated during early G1, ensuring tight cell-cycle control.
Cyclin D-CDK4 complexes integrate mitogenic and antimitogenic signals, acting as key regulators of cell proliferation. This kinase also phosphorylates SMAD3 in a cell-cycle-dependent manner, repressing its transcriptional activity. It forms part of a ternary complex with cyclin D and CDKN1B, which is required for the nuclear translocation and functional activity of the cyclin D-CDK4 complex. These multifaceted roles make it crucial for coordinating cell-cycle progression and responding to external signals that influence cellular growth and division.
| Product Name | Recombinant Human Cyclin-Dependent Kinase 4 (CDK4) Protein (His) |
| Accession | P11802 |
| Purity | Greater than 90% as determined by SDS-PAGE. |
| Host Species | Human |
| Gene | CDK4 |
| Source | E.coli |
| Protein Expression Range | 2-303aa |
| Tag | N-6His |
| Molecular Mass | 37.6kDa |
| Form | Liquid or Lyophilized powder |
| Buffer | Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
| Storage | 1. Store at -20¡«C/-80¡«C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4¡«C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20¡«C/-80¡«C. Protein in lyophilized powder form is stable for up to 12 months at -20¡«C/-80¡«C. |
