Recombinant Human Cytochrome c1, heme protein, mitochondrial (CYC1)

• Purity: Greater than 90% as determined by SDS-PAGE.
• Host Species: Homo sapiens (Human)
• Accession: P08574
• Gene: CYC1
• CAT.NO: B000246
• Expression Systems: E.coli
• Product Name: Recombinant Human Cytochrome c1, heme protein, mitochondrial (CYC1)
• Protein Expression Range: 85-325aa
• Tag: N-terminal GST-tagged
• Molecular Mass: 54.4kDa
• Form: Liquid or Lyophilized powder
• Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Research Area: Transport
• Alternative Names: Complex III subunit 4; Complex III subunit IV; CY1_HUMAN; CYC1; Cytochrome b c1 complex subunit 4 ; Cytochrome b-c1 complex subunit 4; Cytochrome bc1 complex subunit 4 ; Cytochrome c 1; Cytochrome c-1; Cytochrome c1; heme protein; mitochondrial; Ubiquinol cytochrome c reductase complex cytochrome c1 subunit; Ubiquinol-cytochrome-c reductase complex cytochrome c1 subunit; UQCR4
• Target Protein Sequence: SDLELHPPSYPWSHRGLLSSLDHTSIRRGFQVYKQVCASCHSMDFVAYRHLVGVCYTEDEAKELAAEVEVQDGPNEDGEMFMRPGKLFDYFPKPYPNSEAARAANNGALPPDLSYIVRARHGGEDYVFSLLTGYCEPPTGVSLREGLYFNPYFPGQAIAMAPPIYTDVLEFDDGTPATMSQIAKDVCTFLRWASEPEHDHRKRMGLKMLMMMALLVPLVYTIKRHKWSVLKSRKLAYRPPK
• Protein Length: Full Length of Mature Protein
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
• Shelf Life: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.; Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Cytochrome c1 is a component of the ubiquinol-cytochrome c oxidoreductase, also known as the cytochrome b-c1 complex, which is part of the mitochondrial electron transport chain driving oxidative phosphorylation. This multisubunit complex, along with succinate dehydrogenase (complex II) and cytochrome c oxidase (complex IV), transfers electrons from NADH and succinate to molecular oxygen, creating an electrochemical gradient across the inner mitochondrial membrane that powers ATP synthase. The cytochrome b-c1 complex catalyzes electron transfer from ubiquinol to cytochrome c, coupling this reaction with proton translocation. In the Q cycle, protons are consumed and released, and electrons are passed to cytochrome c. Cytochrome c1 transfers electrons from the Rieske protein’s [2Fe-2S] cluster to cytochrome c.