Recombinant Human Cytotoxic T-Lymphocyte Protein 4 (CTLA4) Protein (His), Active

• Purity: Greater than 95% as determined by SDS-PAGE.
• Host Species: Homo sapiens (Human)
• Accession: P16410
• Gene: CTLA4
• CAT.NO: B000254
• Expression Systems: Mammalian cell
• Product Name: Recombinant Human Cytotoxic T-Lymphocyte Protein 4 (CTLA4) Protein (His), Active
• Endotoxin: Less than 1.0 EU/μg as determined by LAL method.
• Protein Expression Range: 36-161aa
• Tag: C-6His
• Molecular Mass: 14.3 kDa
• Form: Lyophilized powder
• Buffer: Lyophilized from a 0.2 μm filtered 1xPBS, pH 7.4
• Storage: 1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use.; 2. Avoid repeated freeze-thaw cycles.; 3. Store working aliquots at 4°C for up to one week.; 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
• Bioactivity: The ED50 as determined by its ability to bind Mouse B7-1 in functional ELISA is less than 20 ng/ml.
• Research Area: Immunology
• Protein Length: Extracellular Domain

CTLA4 is an inhibitory receptor that serves as a key negative regulator of T-cell responses. It has a significantly stronger affinity for its natural B7 family ligands, CD80 and CD86, compared to the affinity of the stimulatory coreceptor CD28 for the same ligands. This high binding affinity allows CTLA4 to effectively suppress T-cell activation and limit immune responses, thereby playing an essential role in maintaining immune homeostasis and preventing excessive immune activation, which could lead to autoimmune disorders.