The alpha subunit of the heteropentameric GABA(A) receptor (GABAAR) forms part of a ligand-gated chloride channel activated by gamma-aminobutyric acid (GABA), the brain’s major inhibitory neurotransmitter. GABAARs are composed of five subunits arranged around a central pore, with GABA binding sites located at alpha-beta subunit interfaces. Upon activation by GABA, GABAARs allow chloride ions to flow across the cell membrane, hyperpolarizing the postsynaptic neuron and reducing its ability to generate action potentials, thereby inhibiting nerve transmission.
Alpha-1-containing GABAARs are primarily synaptic. Receptors with alpha-1 and beta-2 or -3 subunits exhibit synaptogenic activity, with gamma-2 subunits required for rapid synapse formation. GABAARs also function as histamine receptors, with histamine binding at beta subunit interfaces to potentiate GABA responses. Additionally, receptors with alpha, beta, and epsilon subunits enable spontaneous chloride channel activity while maintaining GABA-gated functionality. Alpha-1-mediated plasticity in the orbitofrontal cortex influences action selection, while in the cerebellum, GABAARs may regulate neuronal and glial GABAergic transmission.
| Product Name | Recombinant Human D (DRD2) Protein (His&Myc) |
| Accession | P14416 |
| Purity | Greater than 85% as determined by SDS-PAGE. |
| Host Species | Human |
| Gene | DRD2 |
| Source | E.coli |
| Protein Expression Range | 214-373aa |
| Tag | N-10His&C-Myc |
| Molecular Mass | 25.8 kDa |
| Form | Liquid or Lyophilized powder |
| Buffer | Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
| Storage | 1. Store at -20¡«C/-80¡«C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4¡«C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20¡«C/-80¡«C. Protein in lyophilized powder form is stable for up to 12 months at -20¡«C/-80¡«C. |
