Recombinant Human EGFR Protein (C-6His)

  • Purity: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
  • Host Species: Human
  • Accession: P00533
  • Gene: EGFR
  • CAT.NO: B000099
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Recombinant Human Epidermal Growth Factor Receptor is produced by our Mammalian expression system and the target gene encoding Leu25-Ser645 is expressed with a 6His tag at the C-terminus.

Product Name Recombinant Human EGFR Protein (C-6His)
Accession P00533
Purity Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Endotoxin Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Host Species Human
Gene EGFR
Source Human Cells
Protein Expression Range Leu25-Ser645
Tag C-6His
Molecular Mass 69.6 KDa
Buffer

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Storage

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.

Bioactivity

Loaded Anti-Human EGFR mAb-Fc on Pro A Biosensor, can bind Recombinant Human EGFR-His with an affinity constant of 7.23 nM as determined in BLI assay. (Regularly tested)

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