Recombinant Human EGFR Protein (C-6His)

• Purity: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
• Host Species: Human
• Accession: P00533
• Gene: EGFR
• CAT.NO: B000099
• Product Name: Recombinant Human EGFR Protein (C-6His)
• Endotoxin: Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
• Source: Human Cells
• Protein Expression Range: Leu25-Ser645
• Tag: C-6His
• Molecular Mass: 69.6 KDa
• Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Bioactivity: Loaded Anti-Human EGFR mAb-Fc on Pro A Biosensor, can bind Recombinant Human EGFR-His with an affinity constant of 7.23 nM as determined in BLI assay. (Regularly tested)

The receptor tyrosine kinase EGFR binds ligands from the EGF family to activate signaling cascades that translate extracellular signals into cellular responses. Known ligands include EGF, TGFA/TGF-alpha, AREG, epigen (EPGN), betacellulin (BTC), epiregulin (EREG), and heparin-binding EGF (HBEGF). Ligand binding induces receptor homo- or heterodimerization and autophosphorylation on key cytoplasmic residues, enabling the recruitment of adapter proteins like GRB2, which initiate complex downstream signaling pathways.
EGFR activates at least four major signaling cascades: RAS-RAF-MEK-ERK, PI3K-AKT, PLCgamma-PKC, and STATs. It may also engage the NF-kappa-B pathway. Additionally, EGFR directly phosphorylates proteins such as RGS16, enhancing its GTPase activity to potentially link EGFR and G protein-coupled receptor signaling. It also phosphorylates MUC1, facilitating its interaction with SRC and beta-catenin (CTNNB1).
EGFR promotes cell migration through interaction with CCDC88A/GIV, retaining the receptor at the cell membrane post-ligand stimulation and sustaining signaling for migratory responses. Beyond cellular signaling, EGFR contributes to learning and memory performance and mammalian pain signaling, particularly in long-lasting hypersensitivity. These diverse roles underline EGFR’s critical functions in cellular communication, migration, and broader physiological processes.