EGLN1 is a cellular oxygen sensor that catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins under normoxic conditions. It hydroxylates specific proline residues in the oxygen-dependent degradation (ODD) domains, including the N-terminal (NODD) and C-terminal (CODD) sites of HIF1A, with a preference for the CODD site in both HIF1A and HIF2A. This hydroxylation marks HIFs for proteasomal degradation via the von Hippel-Lindau (VHL) ubiquitination complex. Under hypoxic conditions, EGLN1 activity decreases, allowing HIFs to escape degradation, translocate to the nucleus, heterodimerize with HIF1B, and activate hypoxia-inducible gene expression.
EGLN1, the key isozyme under normoxia, plays a central role in regulating HIF1 stability, influencing processes like retinal and cardiac angiogenesis. It preferentially recognizes substrates via an LXXLAP motif, underlining its specificity and regulatory importance in oxygen-dependent cellular pathways and hypoxia-responsive mechanisms.
| Product Name | Recombinant Human Egl Nine Homolog 1 (EGLN1) Protein (His) |
| Accession | Q9GZT9 |
| Purity | Greater than 90% as determined by SDS-PAGE. |
| Host Species | Human |
| Gene | EGLN1 |
| Source | E.coli |
| Protein Expression Range | 177-426aa |
| Tag | N-6His |
| Molecular Mass | 31.9 kDa |
| Form | Liquid or Lyophilized powder |
| Buffer | Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
| Storage | 1. Store at -20¡«C/-80¡«C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4¡«C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20¡«C/-80¡«C. Protein in lyophilized powder form is stable for up to 12 months at -20¡«C/-80¡«C. |
