Recombinant Human Estrogen Receptor (ESR1) Protein (His)

• Purity: Greater than 90% as determined by SDS-PAGE.
• Host Species: Human
• Accession: P03372
• Gene: ESR1
• CAT.NO: B000092
• Expression Systems: Yeast
• Product Name: Recombinant Human Estrogen Receptor (ESR1) Protein (His)
• Protein Expression Range: 10-595aa
• Tag: N-6His
• Molecular Mass: 67.2kDa
• Form: Liquid or Lyophilized powder
• Buffer: Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
• Storage: 1. Store at -20¡«C/-80¡«C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4¡«C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20¡«C/-80¡«C. Protein in lyophilized powder form is stable for up to 12 months at -20¡«C/-80¡«C.

This nuclear hormone receptor regulates eukaryotic gene expression and influences cellular proliferation and differentiation in target tissues. Ligand binding, typically by estradiol (E2), induces conformational changes that enable receptor activation. The receptor mediates gene transcription through two mechanisms: direct binding as a homodimer to palindromic estrogen response elements (EREs) or interaction with other transcription factors, such as AP-1/c-Jun, c-Fos, ATF-2, Sp1, and Sp3, facilitating ERE-independent signaling.
Ligand-induced activation allows the receptor to associate with multiprotein coactivator complexes via LXXLL motifs, driving transcription. The receptor also engages in mutual transrepression with NF-kappa-B in a cell-type-specific manner, reducing NF-kappa-B DNA binding and suppressing transcription from promoters such as IL6. It can displace NF-kappa-B subunits, such as RELA/p65, and their coregulators from target promoters, including IL8 and CCL2. Additionally, it may synergize with NF-kappa-B to activate transcription, involving shared recruitment to adjacent response elements and coactivator CREBBP.
The receptor activates transcription of genes like TFF1 and mediates membrane-initiated estrogen signaling through kinase cascades. It is critical for MTA1-driven regulation of BRCA1 and BCAS3 expression. Furthermore, it supports neuronal survival during ischemic reperfusion injury in the presence of circulating estradiol, emphasizing its broad physiological and protective roles.