This protein mediates the nuclear export of cellular proteins with a leucine-rich nuclear export signal (NES) and RNAs. In the nucleus, it forms a complex with RANBP3, binding to the NES on its target protein and the active GTP-bound form of RAN (Ran-GTP). The complex docks at the nuclear pore complex (NPC) via nucleoporins. Upon export to the cytoplasm, the complex disassembles, and Ran-GTP is hydrolyzed to Ran-GDP, releasing the cargo. This process relies on the asymmetric distribution of Ran-GTP and Ran-GDP. Additionally, it is involved in transporting U3 snoRNA from Cajal bodies to nucleoli.
| Product Name | Recombinant Human Exportin-1 (XPO1), partial |
| Accession | O14980 |
| Purity | >85% (SDS-PAGE) |
| Host Species | Homo sapiens (Human) |
| Gene | XPO1 |
| Source | E.coli;In Vivo Biotinylation in E.coli;Yeast;Baculovirus;Mammalian cell |
| Tag | Tag type will be determined during the manufacturing process. |
| Form | Lyophilized powder |
| Buffer | Tris/PBS-based buffer, 6% Trehalose. |
| Storage | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
| Alternative Names | Chromosome region maintenance 1 protein homolog; CRM 1; CRM1 homolog; DKFZp686B1823; emb; Exp 1; Exp1; Exportin 1; Exportin-1; Exportin1; XPO 1; xpo1; XPO1_HUMAN |
| Protein Length | Partial |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
| Shelf Life | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. |
| Notes | Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. |
