Tyrosine-protein kinase FGFR3 is a cell-surface receptor for fibroblast growth factors (FGFs) that regulates cell proliferation, differentiation, and apoptosis. It plays a critical role in chondrocyte differentiation, proliferation, and apoptosis, essential for normal skeletal development. FGFR3 regulates osteogenesis and postnatal bone mineralization by osteoblasts and is required for inner ear development. It promotes apoptosis in chondrocytes but can also drive cancer cell proliferation.
Ligand binding activates multiple signaling cascades. FGFR3 phosphorylates PLCG1, CBL, and FRS2. Activation of PLCG1 produces signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. FRS2 phosphorylation recruits GRB2, GAB1, PIK3R1, and SOS1, activating RAS, MAPK1/ERK2, MAPK3/ERK1, and AKT1 pathways. It also regulates vitamin D metabolism. Aberrant FGFR3 signaling due to mutations that cause constitutive kinase activation or impair receptor internalization and degradation can lead to disorders. Overexpression or constitutive activation drives activation of PTPN11/SHP2, STAT1, STAT5A, and STAT5B.
Additionally, the secreted isoform 3 binds FGF1 and FGF2, potentially interfering with FGF signaling. FGFR3’s multifaceted role in development and disease highlights its significance in skeletal biology, cancer, and cellular signaling.
| Product Name | Recombinant Human FGF R3 Protein (C-6His) |
| Accession | P22607 |
| Purity | Greater than 95% as determined by reducing SDS-PAGE. (QC verified) |
| Endotoxin | Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test. |
| Host Species | Human |
| Gene | FGF R3 |
| Source | Human Cells |
| Protein Expression Range | Glu23-Gly375 |
| Tag | C-6His |
| Molecular Mass | 39 KDa |
| Buffer | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
