Recombinant Human HGF R Protein (C-6His)

  • Purity: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
  • Host Species: Human
  • Accession: P08581
  • Gene: HGF R
  • CAT.NO: B000080
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MET is a receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding hepatocyte growth factor (HGF). It regulates key physiological processes, including cell proliferation, scattering, morphogenesis, and survival. Upon ligand binding, MET undergoes autophosphorylation on its intracellular domain, creating docking sites for downstream signaling molecules such as PIK3R1, PLCG1, SRC, GRB2, STAT3, and GAB1.
These interactions activate multiple signaling pathways, including RAS-ERK, PI3K-AKT, and PLCgamma-PKC. The RAS-ERK pathway is linked to morphogenesis, while PI3K-AKT supports prosurvival effects. During embryonic development, MET signaling plays vital roles in gastrulation, neuronal precursor migration, angiogenesis, and kidney formation. In skeletal muscle development, it facilitates the migration of muscle progenitor cells and the proliferation of secondary myoblasts.
In adults, MET contributes to wound healing, organ regeneration, and tissue remodeling. It also supports the differentiation and proliferation of hematopoietic cells and may regulate cortical bone osteogenesis. MET’s diverse roles highlight its importance in development, tissue repair, and maintaining physiological homeostasis.

Product Name Recombinant Human HGF R Protein (C-6His)
Accession P08581
Purity Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Endotoxin Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Host Species Human
Gene HGF R
Source Human Cells
Protein Expression Range Glu25-Thr932
Tag C-6His
Molecular Mass 102.5 KDa
Buffer

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Storage

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.

Bioactivity

Immobilized Recombinant Human HGF-6His at 10μg/ml (100 μl/well) can bind Human HGF R-His: Biotinylated by NHS-biotin prior to testing The ED50 of Recombinant HGF R-His* is 9.47 ug/ml (Regularly tested)

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