Recombinant Human Histone-Lysine N-Methyltransferase Ezh2 (EZH2) Protein (His)

• Purity: Greater than 90% as determined by SDS-PAGE.
• Host Species: Human
• Accession: Q15910
• Gene: EZH2
• CAT.NO: B000014
• Product Name: Recombinant Human Histone-Lysine N-Methyltransferase Ezh2 (EZH2) Protein (His)
• Source: Yeast
• Protein Expression Range: 1-746aa
• Tag: C-6His
• Molecular Mass: 86.8 kDa
• Form: Liquid or Lyophilized powder
• Buffer: Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
• Storage: 1. Store at -20¡«C/-80¡«C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4¡«C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20¡«C/-80¡«C. Protein in lyophilized powder form is stable for up to 12 months at -20¡«C/-80¡«C.

EZH2 is a Polycomb group (PcG) protein and the catalytic subunit of the PRC2/EED-EZH2 complex, which mediates transcriptional repression through histone methylation. It methylates lysine-27 (H3K27) of histone H3, producing mono-, di-, and trimethylated forms (H3K27me1, H3K27me2, and H3K27me3). EZH2 preferentially acts on substrates with lower methylation levels, displaying activity in the order H3K27me0 > H3K27me1 > H3K27me2.
In embryonic stem cells, the PRC2/EED-EZH2 complex is abundant and plays a pivotal role in establishing H3K27me3, crucial for maintaining stem cell identity and driving proper differentiation. It also recruits DNA methyltransferases, integrating histone and DNA methylation systems. Target genes repressed by this complex include HOXC8, HOXA9, MYT1, CDKN2A, and retinoic acid-responsive genes. Beyond histones, EZH2 methylates non-histone proteins such as GATA4 and RORA.
EZH2 regulates the circadian clock by methylating H3K27 at circadian gene promoters, including PER1 and PER2, enabling CRY1/2-mediated transcriptional repression of the CLOCK-BMAL1 heterodimer. This histone modification ensures precise control of circadian rhythm. EZH2’s versatile roles in transcriptional repression and epigenetic regulation highlight its significance in cellular identity, differentiation, and circadian dynamics.