Recombinant Human Immunodeficiency Virus Type 1 Group M Subtype B Envelope Glycoprotein Gp160 (ENV) Protein (His-SUMO)

• Purity: Greater than 90% as determined by SDS-PAGE.
• Host Species: Human immunodeficiency virus type 1 group M subtype B
• Accession: P04578
• Gene: ENV
• CAT.NO: B000090
• Product Name: Recombinant Human Immunodeficiency Virus Type 1 Group M Subtype B Envelope Glycoprotein Gp160 (ENV) Protein (His-SUMO)
• Source: E.coli
• Protein Expression Range: 33-511aa
• Tag: N-6His-SUMO
• Molecular Mass: 69.7 kDa
• Form: Liquid or Lyophilized powder
• Buffer: Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
• Storage: 1. Store at -20¡«C/-80¡«C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4¡«C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20¡«C/-80¡«C. Protein in lyophilized powder form is stable for up to 12 months at -20¡«C/-80¡«C.

In the host endoplasmic reticulum, gp160 predominantly oligomerizes into trimers. It then transits to the host Golgi apparatus, where glycosylation is completed. In the trans-Golgi network, the precursor gp160 undergoes proteolytic cleavage by cellular furin or furin-like proteases, resulting in the formation of gp120 and gp41. This cleavage activates the protein, enabling its role in subsequent biological processes.