Recombinant Human Kelch-Like Ech-Associated Protein 1 (KEAP1) Protein (His)

• Purity: Greater than 90% as determined by SDS-PAGE.
• Host Species: Human
• Accession: Q14145
• Gene: KEAP1
• CAT.NO: B000015
• Expression Systems: Yeast
• Product Name: Recombinant Human Kelch-Like Ech-Associated Protein 1 (KEAP1) Protein (His)
• Protein Expression Range: 1-624aa
• Tag: N-6His
• Molecular Mass: 71.7kDa
• Form: Liquid or Lyophilized powder
• Buffer: Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
• Storage: 1. Store at -20¡«C/-80¡«C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4¡«C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20¡«C/-80¡«C. Protein in lyophilized powder form is stable for up to 12 months at -20¡«C/-80¡«C.

KEAP1 is a substrate-specific adapter for the BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex, playing a critical role in oxidative stress response by targeting NFE2L2/NRF2 for ubiquitination. Under normal conditions, the BCR(KEAP1) complex ubiquitinates and degrades NFE2L2/NRF2, a transcription factor that regulates cytoprotective gene expression.
In oxidative stress, reactive metabolites covalently modify KEAP1’s cysteine residues, inactivating the complex and allowing NFE2L2/NRF2 to translocate to the nucleus, where it promotes stress-response genes. Similarly, in selective autophagy, KEAP1 is sequestered into inclusion bodies via its interaction with SQSTM1/p62, leading to BCR(KEAP1) inactivation and NFE2L2/NRF2 activation. The BCR(KEAP1) complex also ubiquitinates SQSTM1/p62, enhancing its sequestration and degradation activity.
Beyond NRF2, KEAP1 mediates the ubiquitination and proteasomal degradation of other substrates, including BPTF and PGAM5, highlighting its broad regulatory role in stress response and proteostasis.