Recombinant Human Leukocyte Surface Antigen Cd47 (CD47) Protein (hFc), Active

• Purity: Greater than 95% as determined by SDS-PAGE.
• Host Species: Homo sapiens (Human)
• Accession: Q08722
• Gene: CD47
• CAT.NO: B000256
• Expression Systems: Mammalian cell
• Product Name: Recombinant Human Leukocyte Surface Antigen Cd47 (CD47) Protein (hFc), Active
• Endotoxin: Less than 1.0 EU/μg as determined by LAL method.
• Protein Expression Range: 19-139aa
• Tag: C-hFc
• Molecular Mass: 40.8 kDa
• Form: Lyophilized powder
• Buffer: Lyophilized from a 0.2 μm filtered 20 mM PB, 150 mM NaCl, pH 7.4
• Storage: 1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use.; 2. Avoid repeated freeze-thaw cycles.; 3. Store working aliquots at 4°C for up to one week.; 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
• Bioactivity: The ED50 as determined by its ability to bind Human SIRPA in functional ELISA is less than 200 ng/ml.
• Research Area: Immunology
• Protein Length: Partial

This adhesive protein mediates cell-to-cell interactions and functions as a receptor for thrombospondin (THBS1), modulating integrin signaling via the activation of heterotrimeric G proteins. It plays a role in various biological processes, including signal transduction, cardiovascular homeostasis, inflammation, apoptosis, angiogenesis, cellular self-renewal, and immunoregulation. Additionally, it modulates pulmonary endothelin (EDN1) signaling and nitric oxide (NO) signaling, acting as a pressor agent to support blood pressure. In the hippocampus, it is involved in memory formation and synaptic plasticity. It also interacts with SIRPA, preventing dendritic cell maturation and inhibiting cytokine production.